Klenow polymerase is preferable to T4 polymerase in end-labeling reactions
نویسندگان
چکیده
منابع مشابه
End-to-end esophagojejunostomy versus standard end-to-side esophagojejunostomy: which one is preferable?
Abstract Background: End-to-side esophagojejunostomy has almost always been associated with some degree of dysphagia. To overcome this complication we decided to perform an end-to-end anastomosis and compare it with end-to-side Roux-en-Y esophagojejunostomy. Methods: In this prospective study, between 1998 and 2005, 71 patients with a diagnosis of gastric adenocarcinoma underwent total gastrec...
متن کاملPolymerization behavior of Klenow fragment and Taq DNA polymerase in short primer extension reactions.
DNA polymerases amplify DNA fragments through primer extension reactions. However, polymerization behavior of short primers in the primer extension process has not been systematically explored. In this study, we examined the minimal primer length required for primer extension, and the effect of primer length, mismatches and other conditions on DNA polymerization using a non-radioactive method. ...
متن کاملDNA polymerase of bacteriophage T4 is an autogenous translational repressor.
In bacteriophage T4 the protein product of gene 43 (gp43) is a multifunctional DNA polymerase that is essential for replication of the phage genome. The protein harbors DNA-binding, deoxyribonucleotide-binding, DNA-synthesizing (polymerase) and 3'-exonucleolytic (editing) activities as well as a capacity to interact with several other T4-induced replication enzymes. In addition, the T4 gp43 is ...
متن کاملend-to-end esophagojejunostomy versus standard end-to-side esophagojejunostomy: which one is preferable?
abstract background: end-to-side esophagojejunostomy has almost always been associated with some degree of dysphagia. to overcome this complication we decided to perform an end-to-end anastomosis and compare it with end-to-side roux-en-y esophagojejunostomy. methods: in this prospective study, between 1998 and 2005, 71 patients with a diagnosis of gastric adenocarcinoma underwent total gastrect...
متن کاملCloning and expression of T4 DNA polymerase.
The structural gene coding for bacteriophage T4 DNA polymerase (gene 43) has been cloned into inducible plasmid vectors, which provide a source for obtaining large amounts of this enzyme after induction. The T4 DNA polymerase produced in this fashion was purified by an innovative three-step procedure and was fully active.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1990
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/18.19.5909